What is CIS Display™?

Unlike phage, yeast, or mammalian display, CIS Display™ does not involve a biological system. This means that library sizes are less restricted and that larger libraries can be mined effectively.

Originally developed by a team at Isogenica including our current Non-Executive Director Dr. Bill Eldridge, CIS Display™ was first designed to interrogate high-diversity peptide libraries. Over time, this was extended very successfully to increasingly complex small format scaffolds such as Centyrins and VHHs.

Why do we use CIS Display™?

• Display Capacity: Because CIS Display™ is completely cell-free, our synthetic library sizes aren’t limited by a transformation step. This means we can not only create huge libraries, but we can mine them effectively too.
• Scalability: Driven by PCR reactions, we can enrich many different panning arms simultaneously, via our HTP automation. This allows us to experiment with a wider set of conditions such as antigen concentrations, epitope masking, or specific elution to give us the best chance of finding the right conditions to drive towards a TPP.
• Big Data: By seamlessly integrating next-generation sequencing (NGS) analysis, we benefit further from the additional diversity of our libraries, allowing enrichment analysis and identification of rare binders – particularly important with more challenging targets such as membrane proteins.

To learn more, get in touch today.