On-demand webinar: When Hits Don’t Translate – Challenging Membrane Targets from GPCRs to CD3–TCR
About this webinar:
Challenging membrane targets can fail early when target presentation does not reflect the biology teams need to interrogate downstream.
GPCRs, ion channels, multi-pass transmembrane proteins and conformational complexes such as CD3–TCR are highly sensitive to their environment. When conformation, epitope access and mechanism of action are misaligned, discovery campaigns can enrich binders that look promising in selection but fail to translate in functional screening.
This on-demand webinar brings together Isogenica, G.CLIPS and Kactus Bio to explore how teams can reduce risk earlier by aligning target, environment and MOA before critical discovery decisions are made.
Rosie Dawaliby from G.CLIPS introduces the Target–Environment–MOA triad, with a practical framework for discovering and validating conformation- and pathology-sensitive candidates. Anil Kumar from Kactus Bio discusses how nanodisc-based antigen presentation can help preserve native-like structure and improve access to cryptic and conformational epitopes, including for challenging membrane proteins and CD3–TCR complexes.
For teams working on difficult targets, functional screening or VHH antibody discovery, this session provides practical considerations for improving the connection between panning, screening and downstream translation.
What you will learn:
- How target conformation, environment and presentation influence epitope access and binder quality
- How to reduce false positives between panning and functional screening
- How nanodisc-based antigen presentation can support native-like structures for challenging membrane proteins
- Practical considerations for discovery workflows involving GPCRs, ion channels, multi-pass membrane proteins and CD3–TCR complexes.
Working on a challenging membrane target?
Speak to our team about how Isogenica can support your VHH antibody discovery strategy.